HBL-100-CAS9 cell line

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HBL-100-CAS9 (Misidentified source) Order

HBL-100-CAS9 cell line
Catalog#
YC-C013-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HBL-100-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
HBL-100-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HBL-100 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HBL-100-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
HBL-100-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the HBL-100-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HBL-100
STR Info (Cell bank)
Cell Line:HBL-100
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 12121212
D7S820 812812
D16S539 912912
VWA 16161616
TH01 6868
AMEL XXXX
TPOX 8888
CSF1PO 10101010
D12S391 1818
FGA 2525
D2S1338 1824
D21S11 282930.0
D18S51 1616
D8S1179 1215
D3S1358 1416
D6S1043 1214
PENTAE 77
D19S433 1515
PENTAD 1212
D1S1656 1216.3
* Misidentified source
Yoshino et al (2006) notes that the cell line carries a Y chromosome and is therefore unlikely to come from the original donor. HBL-100 was originally reported to come from "Human milk ... obtained by hand expression from a nursing mother 3 days after delivery" (Gaffney, 1982; PMID: 6891286). See also "Misidentified Cell Lines" page on the ATCC website.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
HBL-100-CAS9 cell line
HBL-100-CAS9
Catalog#
YC-C013-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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